1,234 research outputs found
Further correction: Synthesis and characterization of TPGS-gemcitabine prodrug micelles for pancreatic cancer therapy
Get PDFFurther correction for ’Synthesis and characterization of TPGS-gemcitabine prodrug micelles for pancreatic cancer therapy’ by Vaibhav Khare et al., RSC Adv., 2016, 6, 60126-60137
Acridine Orange Fluroscence Study of Lung - Histopathology in Autopsy Cases of Burns
Get PDFBackground: The major cause of death in the burn patients includes multiple organ failure and septicemia but, sometimes the exact cause of death in many fatally burn patients is difficult to detect. Aim: The aim was to study various histopathological changes in lung in the post-mortem cases of burns, by using routine Haematoxylin and Eosin stain (H&E stain). Periodic and Schiff ’s Stain (PAS) stain to study the role of acridine orange fluorescence study, to explore the forensic utility of this study and to find out the relationship between duration of survival and histopathological changes observed. Material & Methods: Total 32 cases of death due to burns were autopsied at mortuary from october 2010 to september 2012, department of Forensic Medicine and Toxicology in our hospital. These were forwarded to Department of Pathology for histopathological examination. Result: In the present study, maximum number of burns cases in 21-30 years of age group & female predominance. Grossly, 19 cases (59.38%) showed congestion while microscopy showed diffuse alveolar damage (34.38%). The sections stained by acridine orange and observed under fluorescent microscope were negative in 28 cases (87.50%) and lightly positive in 04 cases (12.50%). Conclusion: Routine microscopy does help us in getting specific lesions in lung due to burns. But PAS and Acridine orange fluorescence do not add anything further in our knowledge of pathology due to burns. However, none of these add any new tool to resolve any forensic issues of burns. Therefore, microscopy (including PAS and fluorescent), if done would be redundant
Health System Intervention Packages on Improving Coverage of Kangaroo-Mother Care for Preterm or LBW Infants: a Mixed-Methods Systematic Review
Get PDFA new 3D printed radial flow-cell for chemiluminescence detection: Application in ion chromatographic determination of hydrogen peroxide in urine and coffee extracts
Get PDFA new polymer flow-cell for chemiluminescence detection (CLD) has been designed and developed by diverging multiple linear channels from a common centre port in a radial arrangement. The fabrication of radial flow-cell by 3D PolyJet printing and fused deposition modeling (FDM) has been evaluated, and compared with a similarly prepared spiral flow-cell design commonly used in chemiluminescence detectors. The radial flow-cell required only 10 h of post-PolyJet print processing time as compared to ca. 360 h long post-PolyJet print processing time required for the spiral flow-cell. Using flow injection analysis, the PolyJet 3D printed radial flow-cell provided an increase in both the signal magnitude and duration, with an average increase in the peak height of 63% and 58%, peak area of 89% and 90%, and peak base width of 41% and 42%, as compared to a coiled-tubing spiral flow-cell and the PolyJet 3D printed spiral flow-cell, respectively. Computational fluid dynamic (CFD) simulations were applied to understand the origin of the higher CLD signal obtained with the radial flow-cell design, indicating higher spatial coverage near the inlet and lower linear velocities in the radial flow-cell. The developed PolyJet 3D printed radial flow-cell was applied in a new ion chromatography chemiluminescence based assay for the detection of H2O2 in urine and coffee extracts
Comparison of treatment results between holmium laser endourethrotomy and optical internal urethrotomy for urethral stricture
Get PDFSevere renal bleeding caused by a ruptured renal sheath: case report of a rare complication of percutaneous nephrolithotomy
Get PDFBACKGROUND: Percutaneous nephrolithotomy is a minimally invasive intervention for renal stone disease. Complications, which are rare and usually presented as case reports, are diversified as the utilization of the procedure is expanded. The procedure causes less blood loss and less morbidity when compared to open surgical procedures. Yet, there are some reports involving severe bleeding and relevant morbidity during surgery. These are usually related with the surgical technique or experience of the surgeon. Renal sheaths are designed to cause minimal trauma inside the kidney and, to our knowledge, there are no reports presenting the rupture of a sheath causing severe bleeding during the procedure. CASE REPORT: We present an adult patient who had severe bleeding during percutaneous nephrolithotomy due to parenchymal injury caused by a ruptured renal sheath. During retrieval, due probably to rough handling of the equipment, a piece of stone with serrated edges ruptured the tip of the sheath, and this tip caused damage inside the kidney. The operation was terminated and measures were taken to control bleeding. The patient was transfused with a total of 1600 ml of blood, and the stones were cleared in a second look operation. CONCLUSION: Although considered to be a minimally invasive procedure, some unexpected complications may arise during percutaneous nephrolithotomy. After being fragmanted, stone pieces may damage surgical equipment, causing acute and severe harm to the kidney. Surgeons must manipulate the equipment with fine and careful movements in order to prevent this situation
Congenital contractural arachnodactyly (Beals syndrome)
Get PDFCongenital contractural arachnodactyly (Beals syndrome) is an autosomal dominantly inherited connective tissue disorder characterized by multiple flexion contractures, arachnodactyly, severe kyphoscoliosis, abnormal pinnae and muscular hypoplasia. It is caused by a mutation in FBN2 gene on chromosome 5q23. Although the clinical features can be similar to Marfan syndrome (MFS), multiple joint contractures (especially elbow, knee and finger joints), and crumpled ears in the absence of significant aortic root dilatation are characteristic of Beals syndrome and rarely found in Marfan syndrome. The incidence of CCA is unknown and its prevalence is difficult to estimate considering the overlap in phenotype with MFS; the number of patients reported has increased following the identification of FBN2 mutation. Molecular prenatal diagnosis is possible. Ultrasound imaging may be used to demonstrate joint contractures and hypokinesia in suspected cases. Management of children with CCA is symptomatic. Spontaneous improvement in camptodactyly and contractures is observed but residual camptodactyly always remains. Early intervention for scoliosis can prevent morbidity later in life. Cardiac evaluation and ophthalmologic evaluations are recommended
Synthesis and characterization of TPGS-gemcitabine prodrug micelles for pancreatic cancer therapy
Get PDFCorrection for ‘Synthesis and characterization of TPGS–gemcitabine prodrug micelles for pancreatic cancer therapy’ by Vaibhav Khare et al., RSC Adv., 2016, 6, 60126–60137
Approximating k-Forest with Resource Augmentation: A Primal-Dual Approach
Full text linkIn this paper, we study the -forest problem in the model of resource
augmentation. In the -forest problem, given an edge-weighted graph ,
a parameter , and a set of demand pairs , the
objective is to construct a minimum-cost subgraph that connects at least
demands. The problem is hard to approximate---the best-known approximation
ratio is . Furthermore, -forest is as hard to
approximate as the notoriously-hard densest -subgraph problem.
While the -forest problem is hard to approximate in the worst-case, we
show that with the use of resource augmentation, we can efficiently approximate
it up to a constant factor.
First, we restate the problem in terms of the number of demands that are {\em
not} connected. In particular, the objective of the -forest problem can be
viewed as to remove at most demands and find a minimum-cost subgraph that
connects the remaining demands. We use this perspective of the problem to
explain the performance of our algorithm (in terms of the augmentation) in a
more intuitive way.
Specifically, we present a polynomial-time algorithm for the -forest
problem that, for every , removes at most demands and has
cost no more than times the cost of an optimal algorithm
that removes at most demands
Dynamic design: manipulation of millisecond timescale motions on the energy landscape of cyclophilin A
Get PDFProteins need to interconvert between many conformations in order to function, many of which are formed transiently, and sparsely populated. Particularly when the lifetimes of these states approach the millisecond timescale, identifying the relevant structures and the mechanism by which they interconvert remains a tremendous challenge. Here we introduce a novel combination of accelerated MD (aMD) simulations and Markov state modelling (MSM) to explore these ‘excited’ conformational states. Applying this to the highly dynamic protein CypA, a protein involved in immune response and associated with HIV infection, we identify five principally populated conformational states and the atomistic mechanism by which they interconvert. A rational design strategy predicted that the mutant D66A should stabilise the minor conformations and substantially alter the dynamics, whereas the similar mutant H70A should leave the landscape broadly unchanged. These predictions are confirmed using CPMG and R1ρ solution state NMR measurements. By efficiently exploring functionally relevant, but sparsely populated conformations with millisecond lifetimes in silico, our aMD/MSM method has tremendous promise for the design of dynamic protein free energy landscapes for both protein engineering and drug discovery
- …
