4 research outputs found
A new age of leadership in academia: Need for change and innovation during COVID-19
Get PDF漏 The Author(s) 2025.The aim of this study is to explore the role of academic leadership and adaptive leadership on organizational readiness for change. During times of pandemic, adaptive leadership has emerged as a vital leadership discipline along with academic leadership due to uncertainty and sensitivity of situation. In addition, demand of innovative behavior has also increased over the years particularly during Covid-19. The study has been carried out in Higher Education Institutions of Pakistan where the data was collected from deans, directors and head of departments in two phases. Quantitative research strategy was opted for the study. Survey research design was followed to respond objectives of the study. The purpose behind the selection of senior academicians is to draw empirical results from the perspective of all the heads of their relevant departments. The data was collected from seven public sector universities across Pakistan. About 251 responses were found valid. Covariance based SEM was used to analyze the data. Analysis reveals a positive and direct relationship between academic and adaptive leadership and organizational readiness for change and similar results were found by placing innovative behavior as a mediator leading to the acceptance of all developed hypotheses. This study is unique in nature and has implications for leaders in academia in terms of unleashing the potential toward uncertain situation in higher education institutions. Study鈥檚 major limitation include less representation of the Pakistan as whole country as it included Punjab province only for data collection.Unfunde
Heat shock factor 1 inhibition enhances the effects of modulated electro hyperthermia in a triple negative breast cancer mouse model
No full textAbstract Female breast cancer is the most diagnosed cancer worldwide. Triple negative breast cancer (TNBC) is the most aggressive type and there is no existing endocrine or targeted therapy. Modulated electro-hyperthermia (mEHT) is a non-invasive complementary cancer therapy using an electromagnetic field generated by amplitude modulated 13.56聽MHz frequency that induces tumor cell destruction. However, we have demonstrated a strong induction of the heat shock response (HSR) by mEHT, which can result in thermotolerance. We hypothesized that inhibition of the heat shock factor 1 (HSF1) can synergize with mEHT and enhance tumor cell-killing. Thus, we either knocked down the HSF1 gene with a CRISPR/Cas9 lentiviral construct or inhibited HSF1 with a specific small molecule inhibitor: KRIBB11 in vivo. Wild type or HSF1-knockdown 4T1 TNBC cells were inoculated into the mammary gland鈥檚 fat pad of BALB/c mice. Four mEHT treatments were performed every second day and the tumor growth was followed by ultrasound and caliper. KRIBB11 was administrated intraperitoneally at 50聽mg/kg daily for 8聽days. HSF1 and Hsp70 expression were assessed. HSF1 knockdown sensitized transduced cancer cells to mEHT and reduced tumor growth. HSF1 mRNA expression was significantly reduced in the KO group when compared to the empty vector group, and consequently mEHT-induced Hsp70 mRNA upregulation diminished in the KO group. Immunohistochemistry (IHC) confirmed the inhibition of Hsp70 upregulation in mEHT HSF1-KO group. Demonstrating the translational potential of HSF1 inhibition, combined therapy of mEHT with KRIBB11 significantly reduced tumor mass compared to either monotherapy. Inhibition of Hsp70 upregulation by mEHT was also supported by qPCR and IHC. In conclusion, we suggest that mEHT-therapy combined with HSF1 inhibition can be a possible new strategy of TNBC treatment with great translational potential
